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Initial Validation of a Novel Protein Biomarker Panel for Active Pediatric Lupus Nephritis - Hermine I. Brunner, MD

December 13, 2008

Systemic lupus erythematosus (SLE) is an inflammatory autoimmune disease and lupus nephritis(LN) is one of the main determinants of poor prognosis (1). Currently, LN is gauged by measuring circulating and excreted indicators of renal dysfunction, with supporting information from kidney biopsies. The latter constitute the current standard for diagnosing viding a direct assessment of the presence, severity, and activity of LN, and the degree of renal damage (2). Because of the invasive nature of kidney biopsies, clinicians base LN activity and its therapy on the results of urinary protein excretion, urinary sediment, creatinine clearance, and serum albumin. These traditional markers are not accurate in assessing whether active LN is present or not, and none of them is predictive, i.e., can anticipate the course of LN.
Using surface-enhanced laser desorption/ionization timeof-flight mass spectrometry (SELDI-TOF MS) technology, we previously identified a LN urinary protein signature (PS), consisting of eight candidate biomarkers at the mass-to-charge ratios (m/z) of 2.763, 22, 23, 44, 56, 79, 100, and 133 kDa (3). In this study, we present the identification of the specific proteins contained in this PS of children with LN. We further assayed plasma and urine samples of patients with SLE and controls with juvenile idiopathic arthritis (JIA) to investigate the concurrent and predictive validity of the PS proteins to serve as biomarkers of LN activity.

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Source: Alliance for Lupus Research
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